RXMARINE CHEMICAL TAG::
Description::
Application::
Ponceau S is a rapid and reversible stain for detecting protein bands on Western blot membranes and can be used with PVDF, nitrocellulose and cellulose acetate membranes*.
Technical Specifications::
Dose::
Place the blot transfer membrane in a plastic box and rinse it with water three times, 5 minutes each. Stain the membrane with Ponceau S stain for 30 seconds to 1 minute. Destain the membrane with several changes of water for 30 seconds to 1 minute each, then air dry
Product remark::
Industrial Usages::
Useful Area::
Related Information::
Ponceau S is a negative stain which binds to the positively charged amino groups of the protein. It also binds non-covalently to non-polar regions in the protein. (Note: Ponceau S is not suitable for use with nylon membranes.) Ponceau S Staining Solution contains 0.1% Ponceau S (w/v) and 5.0% acetic Acid (w/v).