Detection of Salmonella and Citrobacter in potable water

PRODUCT DATA SHEET
H2S TEST STRIP KIT  
For rapid detection and confirmation of Salmonella and Citrobacter in potable water 
•Easy to perform 
•User-friendly 
•Screening tests 
•Low-cost rapid test 
•Suitable for handling by untrained personnel for monitoring of rural drinking water sources 

Kit contains 
•Glass vial containing H2S test strip 
*Store in a dry place, below 25°C and protect from direct Sunlight. 

Instructions for use 
Carefully open the bottle cap and fill the water sample up to the arrow mark. Quickly re-cap the test bottle and secure it tightly. Shake gently for few minutes to allow the medium to diffuse in water evenly. Incubate at 32 - 37ºC for 18 - 48 hours and take observation.

Observation Broth colour turns to black from initial light amber colour – Salmonella present Water is not suitable for drinking 

Principle There is a need for rapid methods to quickly determine the pathogenic bacteria in water other than coliforms. Monitoring microbiological quality of drinking water also needs examination of water samples for presence of bacterialike Salmonella and Citrobactor freundiiusing rapid kits. H2S test strip kit contains media-impregnated paper strip containingall the essential nutrients for the growth of Salmonella and Citrobactor freundii. Presumptively,presence of these bacteriais indicated by H2S production, resulting in colour change of broth to black from initial light amber colour.

Microbiological parameters (Growth promotion test) Culture characteristics observed after inoculation (10-100 CFU) and incubation at 32 - 37°C for 18 – 48 hours. 

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