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Guanidine is indicated for the reduction of the symptoms of muscle weakness and easy fatigability associated with the myasthenic syndrome of Eaton-Lambert. It is not indicated for treating myasthenia gravis.

Guanidine hydrochloride is also available as an orally administered human drug. Its use is for the treatment of muscle weakness and easy fatigue caused by a rare disease known as the myasthenic syndrome of Eaton-Lambert, which is associated with small-cell carcinoma of the lung.