Product Notes

The formation of the azo-dye using chromotropic acid as a coupling agent was applied to

the determination of five sulphonamides. The spectrophotometric studies as well as microanalysis

of the studied sulphonamides- chromotropic acid azo dyes revealed the existence of the 1:l

coupling product. The absorbance of the formed azo dye is measured at 510 nrn, and has a large

molar absorptivity . 

accuracy and precision over the concentration range used.The formation of the azo-dye using chromotropic acid as a coupling agent was applied to

the determination of five sulphonamides. The spectrophotometric studies as well as microanalysis

of the studied sulphonamides- chromotropic acid azo dyes revealed the existence of the 1:l

coupling product. The absorbance of the formed azo dye is measured at 510 nrn, and has a large

molar absorptivity ( E = 2.87 - 3.29 x lo4 1 mol-'cm-I). Beer's law was obeyed over the

concentration range 0.5-9.0 pg rnl-'. The assay results of pharmaceutical formulations showed good

accuracy and precision over the concentration range used.

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