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HABA dye (4'-hydroxyazobenzene-2-carboxylic acid) is a reagent that enables quick estimation of the mole-to-mole ratio of biotin to protein in a solution. ... The change in absorbance relates to the amount of biotin in the sample by the extinction coefficient of the HABA-avidin complex.

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HABA dye (4'-hydroxyazobenzene-2-carboxylic acid) is a reagent that enables quick estimation of the mole-to-mole ratio of biotin to protein in a solution. ... The change in absorbance relates to the amount of biotin in the sample by the extinction coefficient of the HABA-avidin complex.

Image result for HABA assay

 

HABA dye (4'-hydroxyazobenzene-2-carboxylic acid) is a reagent that enables quick estimation of the mole-to-mole ratio of biotin to protein in a solution. ... The change in absorbance relates to the amount of biotin in the sample by the extinction coefficient of the HABA-avidin complex.

Image result for HABA assay

 

HABA dye (4'-hydroxyazobenzene-2-carboxylic acid) is a reagent that enables quick estimation of the mole-to-mole ratio of biotin to protein in a solution. ... The change in absorbance relates to the amount of biotin in the sample by the extinction coefficient of the HABA-avidin complex.